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fak  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology fak
Effect of 4-HC on the basal activation of <t>FAK</t> and Rac-1 . A . Analysis of samples immunoprecipitated with anti-FAK antibody showed that basal tyrosine phosphorylation of FAK (upper panel) was decreased by 4-HC. The same membrane was stripped and reprobed with anti-paxillin and <t>anti-FAK</t> <t>antibodies.</t> We found no changes on the FAK-bound paxillin levels (middle panel). One representative experiment from 3 is shown. B . The active form of Rac-1 was purified using pull-down assays and then probed with anti-Rac-1 antibody (upper panel). Detection of Rac-1 from total lysates (lower panel) showed that the difference in the amount of active Rac-1 is not associated to changes on its expression. One representative experiment from 2 is shown.
Fak, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fak+h+1/pmc02429896-50-10-26?v=Santa+Cruz+Biotechnology
Average 96 stars, based on 1 article reviews
fak - by Bioz Stars, 2026-07
96/100 stars
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pmcherry-c1-fak-ha-v744g (plasmid #35040)  (Addgene inc)
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Standard format: Plasmid sent in bacteria as agar stab
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pmcherry-c1-fak-ha (plasmid #35039)  (Addgene inc)
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Effect of 4-HC on the basal activation of FAK and Rac-1 . A . Analysis of samples immunoprecipitated with anti-FAK antibody showed that basal tyrosine phosphorylation of FAK (upper panel) was decreased by 4-HC. The same membrane was stripped and reprobed with anti-paxillin and anti-FAK antibodies. We found no changes on the FAK-bound paxillin levels (middle panel). One representative experiment from 3 is shown. B . The active form of Rac-1 was purified using pull-down assays and then probed with anti-Rac-1 antibody (upper panel). Detection of Rac-1 from total lysates (lower panel) showed that the difference in the amount of active Rac-1 is not associated to changes on its expression. One representative experiment from 2 is shown.

Journal: Cancer Cell International

Article Title: Reduced paxillin expression contributes to the antimetastatic effect of 4-hydroxycoumarin on B16-F10 melanoma cells

doi: 10.1186/1475-2867-8-8

Figure Lengend Snippet: Effect of 4-HC on the basal activation of FAK and Rac-1 . A . Analysis of samples immunoprecipitated with anti-FAK antibody showed that basal tyrosine phosphorylation of FAK (upper panel) was decreased by 4-HC. The same membrane was stripped and reprobed with anti-paxillin and anti-FAK antibodies. We found no changes on the FAK-bound paxillin levels (middle panel). One representative experiment from 3 is shown. B . The active form of Rac-1 was purified using pull-down assays and then probed with anti-Rac-1 antibody (upper panel). Detection of Rac-1 from total lysates (lower panel) showed that the difference in the amount of active Rac-1 is not associated to changes on its expression. One representative experiment from 2 is shown.

Article Snippet: Antibodies against paxillin (sc-5574 rabbit polyclonal), β-tubulin (D-10 mouse monoclonal), FAK (H-1 mouse monoclonal), and phosphotyrosine (PY20 mouse monoclonal) as well as siRNAs were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Activation Assay, Immunoprecipitation, Phospho-proteomics, Membrane, Purification, Expressing